A Brownian Dynamics Study of the Initial Stages of Hen Egg-White Lysozyme Adsorption at a Solid Interface
نویسندگان
چکیده
We investigate the initial stages of hen egg-white lysozyme (HEWL) adsorption at a charged solid interface using Brownian Dynamics simulation. The protein is modeled at the atomistic level and the adsorption surface is represented by a planar array of positively charged sites. Adsorption reactions are simulated at neutral pH and at low and high salt concentrations. We find that the HEWL, which has a net positive charge, can adsorb onto the positively charged surface. The results are consistent with an electrostatically driven adsorption process. While the orientational distribution of the adsorbed protein is nonuniform, there is no dominant patch composed of two or more oppositely charged residues. The electric field distribution around the protein is the best predictor of the adsorption behavior.
منابع مشابه
Study the Interaction of Ni Complex of Tetradentate Schiff Base Ligand with HEN Egg White Lysozyme
AbstractInteraction of Ni complex(Salen= N, N´-ethylene bis(salicylideneimine)) with hen egg-white lysozyme (HEWL) was studied by absorption spectroscopy, competitive binding study and thermal denaturation study. The protein binding affinity of Ni complex was found to be (3.0×103M−1). The binding plot obtained from the absorption titration data gives a binding constant of 2.4 (± 0.3)×103 M...
متن کاملInhibition of Amyloid Fibrils Formation from Hen Egg White Lysozyme by Satureia Hortensis Extract and its Effect on Learning and Spatial Memory of Rats
Background & Aims: Alzheimer's disease is a neurodegenerative disorder characterized by the abnormal aggregation of amyloid-β plaques in the brain. Although several studies have been done for finding effective medicines in the treatment of this disease, a drug that inhibits amyloid β aggregation and ameliorates the disorder has not been approved so far. One important therapeutic approach is use...
متن کاملEffect of Cinnamomum Verum Extract on the Amyloid Formation of Hen Egg-white Lysozyme and Study of its Possible Role in Alzheimer’s Disease
Introduction: Diagnosing and treating diseases associated with amyloid fibers remain a great challenge despite of intensive research carried out. One important approach in the development of therapeutics is the use of herbal extracts which are rich in aromatic small molecules. Cinnamomum verum extract (CE) contains proanthocyanidin and cinnamaldehyde, which have been suggested to be capab...
متن کاملMultiprotein Interactions during Surface Adsorption: a Molecular Dynamics Study of Lysozyme Aggregation at a Charged Solid Surface
Multiprotein adsorption of hen egg white lysozyme at a model charged ionic surface is studied using fully atomistic molecular dynamics simulations. Simulations with two, three, and five proteins, in various orientations with respect the surface, are performed over a 100 ns time scale. Mutated proteins with point mutations at the major (Arg128 and Arg125) and minor (Arg68) surface adsorption sit...
متن کاملElectrochemical behaviour of hen-egg-white lysozyme at the polarised water/1,2-dichloroethane interface.
The electrochemical behaviour of hen-egg-white lysozyme (HEWL) was studied at the polarized water/1,2-dichloroethane interface. The voltammetric ion-transfer response was found to be dependent on the pH and ionic strength of the aqueous phase solution and also on the nature of the organic phase electrolyte anion. The current-pH behaviour of HEWL was dominated by the charge of the biomolecule at...
متن کامل